Microemulsion electrokinetic chromatographic determination of bufadienolides in toad venom and in traditional Chinese medicine |
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Authors: | Luo Xingping Zhai Zongde Zhao Yanfang Chen Liren Li Yongmin |
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Affiliation: | (1) Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, People’s Republic of China;(2) The Graduate School of Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China;(3) Gansu Province Institute for Drug Control, Lanzhou, 730000, People’s Republic of China |
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Abstract: | A microemulsion electrokinetic chromatographic (MEEKC) method has been developed and validated for determination of resibufogenin and cinobufagin in toad venom and in traditional Chinese medicine prepared from the venom. The MEEKC method involved use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent, and butan-1-ol as co-solvent. To improve the separation, the effect of temperature and running buffer pH were evaluated. The optimized conditions (heptane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w), and 10 mmol L−1 sodium tetraborate buffer, pH 9.2, and 298 nm as the detection wavelength) enabled useful and repeatable separation of the analytes. |
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Keywords: | Microemulsion electrokinetic chromatography Toad venom Resibufogenin Cinobufagin |
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