Arsenic speciation in clinical samples: urine analysis using fast micro-liquid chromatography ICP-MS |
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Authors: | Email author" target="_blank">Jackie?MortonEmail author Elizabeth?Leese |
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Institution: | (1) Health and Safety Laboratory, Harpur Hill, Buxton, Derbyshire, SK17 9JN, UK |
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Abstract: | Arsenic speciation is a subject that is developing all the time both from improvements in analytical techniques and from increases
in toxicological understanding. Despite speciation methods being widely developed, arsenic speciation is not routinely offered
as an analysis in clinical laboratory. The work in this paper describes a simple routine method for arsenic speciation that
could be easily implemented in clinical laboratories. The method described, a new, fast analytical method for arsenic speciation,
is reported using micro-liquid chromatography hyphenated to an inductively coupled plasma mass spectrometer (μLC-ICP-MS).
The method uses a low-pressure delivery six-port valve with a 5 cm anion exchange column, which allows a fully resolved separation
of five arsenic species (arsenobetaine AB], arsenite As3+], arsenate As5+], mono-methylarsonic acid MMA5+] and dimethylarsinic acid DMA5+]) in urine in just 6 min. This fast analytical method offers an arsenic speciation method that is feasible for a laboratory
that does not have the capability for a dedicated arsenic speciation LC-ICP-MS instrument. The micro-LC system is small, easy
to install and is fully integrated with the ICP-MS software. The results reported here are from urine samples from 65 workers
in a semiconductor work providing a sample for their routine biological monitoring to assess workplace exposure. Control samples
from 20 unexposed people were also determined. Results show that the semiconductor workers exhibit very low levels of arsenic
in their urine samples, similar to the levels in the controls, and thus are not significantly exposed to arsenic. Care must
be taken when interpreting urinary arsenic species results because it is not always possible to differentiate between dietary
and other external sources of exposure. |
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