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| 水热法制备质谱专用提取多肽的金属螯合纳米磁珠 | |
| 引用本文： | 王娜,董方霆,张学敏,李爱玲,王杰,王红霞,李萍,吴胜明,杨晓虹.水热法制备质谱专用提取多肽的金属螯合纳米磁珠[J].化学学报,2007,65(4):344-348. |
| 作者姓名： | 王娜 董方霆 张学敏 李爱玲 王杰 王红霞 李萍 吴胜明 杨晓虹 |
| 作者单位： | 1. 吉林大学药学院,长春130021 2. 国家生物医学分析中心,北京,100850 3. 吉林大学药学院,长春,130021 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 应用水热法合成质谱专用提取血清多肽的金属螯合纳米磁珠, 可用于质谱对血清中多肽分布情况的研究, 获得血清多肽谱. 对磁珠进行透射电镜(TEM), 原子力显微镜(AFM)和傅立叶红外光谱FT-IR的表征, 显示该粒子粒径在70～90 nm. 并通过质谱验证该金属螯合磁珠能有效提取血清中的多肽, 该磁珠为质谱进行疾病诊断解决样品制备的技术难题, 具有广阔应用前景. |
| 关 键 词： | 热水解法 质谱 多肽 金属螯合磁珠 |
| 收稿时间： | 2006-4-11 |
| 修稿时间： | 2006-04-112006-10-27 |
Hydrothermal Synthesis of Metal-Chelated Magnetic Nanobeads for Exclusive Use of Peptides Extraction by Mass Spectrum |
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| WANG,Na,DONG,Fang-Ting,ZHANG,Xue-Min,LI,Ai-Ling,WANG,Jie,WANG,Hong-Xia,LI,Ping,WU,Sheng-Ming,YANG,Xiao-Hong.Hydrothermal Synthesis of Metal-Chelated Magnetic Nanobeads for Exclusive Use of Peptides Extraction by Mass Spectrum[J].Acta Chimica Sinica,2007,65(4):344-348. | |
| Authors: | WANG Na DONG Fang-Ting ZHANG Xue-Min LI Ai-Ling WANG Jie WANG Hong-Xia LI Ping WU Sheng-Ming YANG Xiao-Hong |
| Institution: | 1. Pharmaceutical Department of Jilin University, Changchun 130021;2. National Center of Biomedical Analysis, Beijing 100850 |
| Abstract: | A novel hydrothermal route has been developed to produce metal-chelated magnetic nanobeads which help to study serum peptides profiles by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrum. The obtained particles were analyzed by TEM, AFM and FT-IR techniques, and it showed that the average particle size ranged between 70～90 nm. It is validated by mass spectrum that the metal-chelated magnetic nanobeads can extract serum peptides in an effective way. These beads are very useful in the clinic studies on serum preparation for mass spectrum in future. |
| Keywords: | hydrothermal method mass spectrum peptide metal-chelated magnetic nanobead |
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