Micellar electrokinetic chromatography (MEKC) separation of furanonaphthoquinones from Tabebuia impetiginosa

The separation of nine furanonaphthoquinones by micellar electrokinetic chromatography (MEKC) is described. The running electrolytes used in this method were 0.03 M sodium dodecyl sulphate (SDS) in 0.09 M borate buffer (pH 9) containing 10% methanol, with an applied voltage of 20 kV. Application of this technique in the determination of the main furanonaphthoquinones, 5-hydroxy-2-(1-hydroxyethyl)naphtho2,3-b]furan-4,9-dione, 8-hydroxy-2-(1-hydroxyethyl)naphtho2,3-b]furan-4,9-dione, and 2-(1-hydroxyethyl)naphtho2,3-b]furan-4,9-dione, of Tabebuia impetiginosa is demonstrated in this paper.