| 精吡氟禾草灵酶联免疫吸附分析方法研究 |
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| 引用本文： | 鲍华军,武霓,施海燕,王鸣华. 精吡氟禾草灵酶联免疫吸附分析方法研究[J]. 分析科学学报, 2010, 26(6): 665-668 |
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| 作者姓名： | 鲍华军 武霓 施海燕 王鸣华 |
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| 作者单位： | 南京农业大学植物保护学院农药科学系,农业部作物病虫害监测与防控重点开放实验室,江苏南京,210095;南京农业大学植物保护学院农药科学系,农业部作物病虫害监测与防控重点开放实验室,江苏南京,210095;南京农业大学植物保护学院农药科学系,农业部作物病虫害监测与防控重点开放实验室,江苏南京,210095;南京农业大学植物保护学院农药科学系,农业部作物病虫害监测与防控重点开放实验室,江苏南京,210095 |
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| 基金项目： | 贵州大学绿色农药与农业生物工程教育部重点实验室开放基金(No.2008GDGP0102) |
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| 摘 要： | 建立了定量测定精吡氟禾草灵的间接竞争酶联免疫吸附分析方法(ic-ELISA)。将精吡氟禾草灵在碱性条件下水解制得半抗原,再将半抗原与蛋白质偶联形成抗原后免疫新西兰大白兔,获得多克隆抗体。对其进行条件优化后,得到了精吡氟禾草灵的ic-ELISA方法的标准曲线。该方法的Ic50为0.553mg/L。检出限为0.0062mg/L。方法对其他芳氧苯氧基丙酸酯类除草剂没有明显交叉反应。精吡氟禾草灵在样品中的回收率为86.80%～103.41%,变异系数为3.96%～12.32%。表明本研究建立的精吡氟禾草灵的ELISA方法符合农药残留分析的要求。
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| 关 键 词： | 精吡氟禾草灵 半抗原 多克隆抗体 酶联免疫吸附分析 |
An Enzyme-Linked Immunosorbent Assay for Fluazifop-p-butyl |
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| BAO Hua-jun,WU Ni,SHI Hai-yan,WANG Ming-hua. An Enzyme-Linked Immunosorbent Assay for Fluazifop-p-butyl[J]. Journal of Analytical Science, 2010, 26(6): 665-668 |
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| Authors: | BAO Hua-jun WU Ni SHI Hai-yan WANG Ming-hua |
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| Affiliation: | BAO Hua-jun,WU Ni,SHI Hai-yan,WANG Ming-hua (Department of Pesticide Science,College of Plant Protection,Nanjing Agricultural University,Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects,Ministry of Agriculture,Nanjing210095) |
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| Abstract: | An indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)for the determination of the herbicide fluazifop-p-butyl was developed.The hapten was synthesized by hydrolyzing fluazifop-p-butyl,and conjugated with the carrier protein.The polyclonal antibodies were obtained by immunizing New Zealand rabbits with hapten-protein conjugates.The optimized ic-ELISA showed Ic50value was 0.553mg/L,with a detection limit of 0.0062mg/L.The cross-reactivities of analogs of fluazifop-p-butyl tested were less than 0... |
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| Keywords: | Fluazifop-p-butyl Hapten Polyclonal antibody Enzyme-linked immunosovbent assay |
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