Fluorophore-encapsulated solid-supported bilayer vesicles: a method for studying membrane permeation processes |
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Authors: | Williams Thomas L Vareiro Margarida M L M Jenkins A Toby A |
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Affiliation: | Department of Chemistry, University of Bath, Bath BA2 7AY, United Kingdom. |
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Abstract: | This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents. |
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