| Abstract: | High resolution could be achieved for the enantiomers of acidic drugs, namely, sulindac, fenoprofen, ketoprofen, warfarin, and hexobarbital, in a buffer of pH 3 by the simultaneous addition of uncharged and charged β-cyclodextrin derivatives. The interaction of the analytes with the anionic sulfobutyl ether β-cyclodextrin provides the analytes with an adequate electrophoretic mobility whereas the interaction with various neutral β-cyclodextrins generates high enantioselectivity. Five neutral cyclodextrins, the native β-cyclodextrin, as well as methyl-, dimethyl-, trimethyl- and hydroxypropyl-β-cyclodextrin, were tested to enhance the enantioselectivity of the electrophoretic system. High resolution values and the shortest analysis times for the five drugs tested were achieved in a buffer made of 100 mM phosphoric acid adjusted to pH 3 with triethanolamine and containing dimethyl- or trimethyl-β-cyclodextrin in addition to sulfobutyl ether β-cyclodextrin. |
