| 碱溶性茶多糖的提取及其分析 |
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| 引用本文： | 陈小强,周瑛,叶阳,成浩,尹军峰.碱溶性茶多糖的提取及其分析[J].应用化学,2008,25(12):1496-0. |
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| 作者姓名： | 陈小强 周瑛 叶阳 成浩 尹军峰 |
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| 作者单位： | 中国农业科学院茶叶研究所，农业部茶及饮料作物产品加工与质量控制重点开放实验室 |
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| 基金项目： | 浙江省自然科学基金
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| 摘 要： | 在煎煮后的低档绿茶茶渣中提取碱溶性茶多糖,经HPGPC-ELSD法分析其含有3种均一性多糖组分,依出峰顺序其质量分数约为5.96%、78.99%和15.05%;GC-MS法测得组成碱溶性茶多糖的6种单糖:鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖,并分析了其摩尔比例.紫外-可见吸收光谱分析显示,碱溶性茶多糖在250～290 nm区段有"波浪状"吸收.红外光谱表征了碱溶性茶多糖的特征,圆二色谱分析了其水溶液的cotton效应.结果表明,在190 nm有正cotton效应,在203 nm有负cotton效应.
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| 关 键 词： | 碱溶性茶多糖 提取 分析 |
| 收稿时间： | 2009-06-29 |
| 修稿时间： | 2009-06-29 |
Extraction and Analysis of Alkali-soluble Tea Polysaccharides |
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| CHEN Xiao-Qiang,ZHOU Ying,YE Yang,CHENG Hao,YIN Jun-Feng.Extraction and Analysis of Alkali-soluble Tea Polysaccharides[J].Chinese Journal of Applied Chemistry,2008,25(12):1496-0. |
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| Authors: | CHEN Xiao-Qiang ZHOU Ying YE Yang CHENG Hao YIN Jun-Feng |
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| Institution: | CHEN Xiao-Qianga*,ZHOU Yingb,YE Yanga,CHENG Haoa,YIN Jun-Fenga(aTea Research Institute of China Academy of Agricultural Sciences,Key Laboratory of Machining , Quality Control of Tea , Beverage Plants,Ministry of Agriculture,Hangzhou 310008,bCollege of Chemical Engineering , Materials Science,Zhejiang University of Technology,Hangzhou) |
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| Abstract: | Alkali-soluble tea polysaccharides(ATPS) were extracted from tea residue after decocting of low-grade green tea.There were 3 kinds of homogeneous constituents whose mass fractions were about 5.96%,78.99% and 15.05%(according to the retention time) analyzed by means of HPGPC-ELSD in ATPS.The polysaccharides were made up of six kinds of monosaccharide residues(rhamnose,arabinose,xylose,mannose,glucose and galatose) determined by means of GC-MS as well as their molar ratio.CD spectra of ATPS in water showed a ... |
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| Keywords: | alkali-soluble tea polysaccharides(ATPS) extraction analysis |
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