| 高效液相色谱梯度洗脱法测定唐威胶囊中的盐酸小檗碱及葛根素 |
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| 引用本文： | 王玉杰,杨秀娟,张玉华,赵万国.高效液相色谱梯度洗脱法测定唐威胶囊中的盐酸小檗碱及葛根素[J].色谱,2000,18(5):465-467. |
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| 作者姓名： | 王玉杰 杨秀娟 张玉华 赵万国 |
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| 作者单位： | 1. 长春市药品检验所,吉林,长春,130061
2. 吉林省农安县妇幼保健站,吉林,长春,130200 |
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| 摘 要： | 采用反相高效液相色谱梯度洗脱法,用ODS柱,以甲醇-醋酸-三乙胺-水为流动相,在230 nm处同时测定唐威胶囊中盐酸小檗碱及葛根素的含量。平均添加回收率(n=5)和RSD值分别为98.2%和1.6%(盐酸小檗碱);97.5%和1.8%(葛根素)。
关键词：
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| 关 键 词： | 反相高效液相色谱法 唐威胶囊 盐酸小檗碱 葛根素 梯度洗脱 |
| 修稿时间： | 1999-09-20 |
Determination of Beberine Hydrochloride and Puerarin in Tangwei Capsules by RP-HPLC of Gradient Elution Method |
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| WANG Yu-jie,YANG Xiu-juan,ZHANG Yu-hua,ZHAO Wan-guo.Determination of Beberine Hydrochloride and Puerarin in Tangwei Capsules by RP-HPLC of Gradient Elution Method[J].Chinese Journal of Chromatography,2000,18(5):465-467. |
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| Authors: | WANG Yu-jie YANG Xiu-juan ZHANG Yu-hua ZHAO Wan-guo |
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| Institution: | Changchun Municipal Institute for Drug Control, Changchun 130061, China. |
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| Abstract: | A reversed-phase high performance liquid chromatographic gradient elution method was developed for the determination of beberine hydrochloride and puerarin in Tangwei capsules. Separation was obtained by using Hypersil ODS (150 mm x 4.6 mm i.d., 5 microns) column and mobile phase of methanol and acetic acid, triethylamine water solution. Detection was performed with UV detector at 230 nm. The column temperature was 35 degrees C. The calibration curves were linear within the range of 0.18 microgram-1.66 micrograms for beberine hydrochloride (n = 5, r = 0.9999) and 0.09 microgram-0.89 microgram for puerarin (n = 5, r = 0.9998). The recovery and RSD of beberine hydrochloride were 98.2% and 1.6% and those of puerarin were 97.5% and 1.8%, respectively. |
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| Keywords: | high performance liquid chromatography Tangwei capsules beberine hydrochloride puerarin gradient elution |
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