PHOSPHORYLATED REACTION OF SNAKE MUSCLE FRUCTOSE 1,6-BISPHOSPHATASE IN ITS REVERSE REACTION |
| |
作者姓名: | 胡国富 许根俊 |
| |
作者单位: | Shanghai Institute of Biochemistry,Academia Sinica,Shanghai Institute of Biochemistry,Academia Sinica |
| |
基金项目: | Project supported by the National Natural Science Foundation of China. |
| |
摘 要: | A phosphoryl enzyme intermediate was observed in the hydrolysis of FruP_2 catalyzed by snake muscleFruP_(2ase) based on the fact that the formation rate of F6P is faster than that of inorganic phosphatewhen the reaction mixture contains a phosphoryl acceptor other than water. A covalently bound phosphateon the enzyme was isolated from the reverse reaction. This intermediate may relate to the process of theforward catalytic reaction by the enzyme.
|
本文献已被 CNKI 等数据库收录! |