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Determination of enterostatin in human cerebrospinal fluid by capillary electrophoresis with laser induced fluorescence detection |
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| Authors: | Shulin Zhao Chandan Prasad Hugh J. F. Robertson Y.-M. Liu |
| Affiliation: | Department of Chemistry, Jackson State University, 1400 J. R. Lynch Street, Jackson, MS 39217, USA, US Department of Medicine, Louisiana State University Medical Center, 1542 Tulane Ave., New Orleans, LA 70112, USA, US Department of Radiology, Louisiana State University Medical Center, 1542 Tulane Ave., New Orleans, LA 70112, USA, US |
| Abstract: | A capillary electrophoresis (CE) method with laser induced fluorescence (LIF) detection is described for quantification of enterostatin (Val-Pro-Asp-Pro-Arg), a pentapeptide involved in appetite regulation and insulin secretion. Enterostatin and two other pentapeptides belonging to the enterostatin family (i.e. Ala-Pro-Gly-Pro-Arg and Val-Pro-Gly-Pro-Arg) were well separated from each other. The peptides were fluorescently tagged with naphthalene-2,3- dicarboxaldehyde (NDA) and separated by micellar electrokinetic chromatography (MEKC) in the presence of methanol as an organic modifier. Coupled with LIF detection, the method had a detection limit of 4.8 × 10–6 M for enterostatin. The relative standard deviation was to be 4.0% from five determinations of enterostatin at 37.2 μM in a human cerebrospinal fluid (CSF) sample. Twenty-three human CSF samples were analyzed. The level of enterostatin ranged from 24 μM to 51 μM with a mean (± SEM) value of 41.7 ± 2.0 μM. |
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