Extraction of plant proteins for two-dimensional electrophoresis |
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| Authors: | F Granier |
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| Affiliation: | Laboratoire de Génétique des Systèmes Végétaux, La Ferme du Moulon, Gif-sur-Yvette, France. |
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| Abstract: | Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Tris-buffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membrane proteins in addition to a recovery of soluble proteins, and (iii) a trichloroacetic acid-acetone procedure allowing the direct precipitation of total proteins. |
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