A MIP-based flow-through fluoroimmunosensor as an alternative to immunosensors for the determination of digoxin in serum samples |
| |
Authors: | Gema Paniagua González Pilar Fernández Hernando Jesús Senén Durand Alegría |
| |
Institution: | (1) Departamento de Ciencias Analíticas, Facultad de Ciencias, Universidad Nacional de Educación a Distancia (UNED), 28040 Madrid, Spain |
| |
Abstract: | This work reports a comparative study of two automated flow-through fluorosensors for the determination of digoxin in serum
samples: an immunosensor with an anti-digoxin polyclonal antibody as the reactive phase permanently immobilised on controlled-pore
glass and a sensor with a selective reaction system based on a methacrylic molecularly imprinted polymer (MIP) synthesised
by bulk polymerisation. The variables affecting the sensitivity and dynamic range of the sensors (e.g. the carrier and elution
solutions, flow rates, pH and reagent concentrations) were optimized, and the binding characteristics of their reactive phases
were compared in a competitive fluorescent assay. Digoxin was reproducibly determined by both sensors at the milligram per
litre level (detection limit = 1.20 × 10−3 mg L−1 and RSD = 4–7% for the immunosensor; detection limit = 1.7 × 10−5 mg L−1 and RSD = 1–2% for the MIP sensor). No cross-reactivity with digoxin-related compounds was seen for either sensor at a digoxin/interferent
ratio of 1:100. The lifetime of the immunosensor was about 50 immunoassays; its shelf life, when unused, is about 3 months.
The lifetime of the MIP sensor was over 18 months. Both sensors were used to determine the digoxin concentration of human
serum samples with satisfactory results. |
| |
Keywords: | Digoxin Permanent immobilisation Molecular imprinting Flow-through fluorosensor Serum analysis |
本文献已被 SpringerLink 等数据库收录! |