Use of rotofor preparative isoelectrofocusing cell in protein purification procedure |
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| Authors: | Antonio Ayala Juan Parrado Alberto Machado |
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| Institution: | (1) Departamento de Bioquimica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain |
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| Abstract: | The Rotofor cell is a preparative isoelectric focusing (IEF) apparatus, in which IEF is performed entirely in free solution.
Electrofocusing in Rotofor cell has been described as well-suited for use at any stage of a purification scheme. However,
it has some important limitations in resolving complex mixtures of proteins. This paper describes the advantages and disadvantages
of using the Rotofor cell in purification protocols. |
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| Keywords: | Rotofor Superoxide dismutase 6-phosphogluconate dehydrogenase |
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