| 磁纳米探针检测人绒毛膜促性腺激素 |
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| 引用本文： | 刘儒平,刘军涛,王蜜霞,罗金平,刘春秀,蔡新霞.磁纳米探针检测人绒毛膜促性腺激素[J].分析化学,2009,37(12). |
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| 作者姓名： | 刘儒平 刘军涛 王蜜霞 罗金平 刘春秀 蔡新霞 |
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| 作者单位： | 中国科学院电子学研究所传感技术联合国家重点实验室,北京,100190;中国科学院研究生院,北京,100080 |
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| 基金项目： | 国家863计划项目，国家自然科学基金项目，中科院创新项目，中科院电子所创新项目 |
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| 摘 要： | 采用链霉亲和素包被磁纳米粒子,将生物素标记的特异性抗体偶联在磁纳米粒子上,制备出高特异性的磁纳米探针;利用此探针对人绒毛膜促性腺激素(HCG)进行测定,建立了定量检测蛋白类激素的化学发光分析方法.利用紫外可见分光光度计、透射电镜及动态光散射仪对磁纳米探针进行表征,同时对化学发光实验条件进行优化.在2×10~(-4) mol/L鲁米诺、8×10~(-4) mol/L H_2O_2, pH=13的优化条件下,将磁纳米探针用于HCG的定量检测.结果表明,所测发光强度与待测HCG浓度之间线性相关,相关系数r为0.9924,线性检测范围由常规板式ELISA的5～200 μg/L扩展到0.5～250 μg/L;相对标准偏差为3.82%.采用本方法和常规ELISA法同时对34份人血清标本HCG进行测试,两者相关性良好.利用制备的磁纳米探针定量测定微量蛋白类激素,具有灵敏、高效、快捷、检测范围宽等优点,有望应用于其它微量蛋白的检测.
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| 关 键 词： | 化学发光免疫分析法 磁纳米探针 动态光散射 人绒毛膜促性腺激素 |
Human Chorionicgonadotropin Measurement Method Based on Magnetic Nanoparticle Probes |
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| LIU Ru-Ping,LIU Jun-Tao,WANG Mi-Xia,LUO Jin-Ping,LIU Chun-Xiu,CAI Xin-Xia.Human Chorionicgonadotropin Measurement Method Based on Magnetic Nanoparticle Probes[J].Chinese Journal of Analytical Chemistry,2009,37(12). |
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| Authors: | LIU Ru-Ping LIU Jun-Tao WANG Mi-Xia LUO Jin-Ping LIU Chun-Xiu CAI Xin-Xia |
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| Institution: | LIU Ru-Ping1,2,LIU Jun-Tao1,WANG Mi-Xia1,LUO Jin-Ping1,LIU Chun-Xiu1,CAI Xin-Xia1,2 1(State Key Laboratory of Transducer Technology,Institute of Electronics Chinese Academy of Sciences,Beijing 100190) 2(Graduate School of Chinese Academy of Sciences,Beijing 100190) |
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| Abstract: | The magnetic nanoparticle probe was prepared by specifically connecting the streptavidin-conjugated magnetic nanoparticles and the antibody of analyte via the strong streptavidin-biotin interaction.Based on the magnetic nanoparticle probes,the concentration of human chorionicgonadotropin(HCG) was detected and a new CL method for of hormone was further established.The performances of the magnetic nanoparticle probes were characterized by UV-Vis spectrometry,transmission electron microscopy and dynamic light ... |
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| Keywords: | Chemiluminescence immunoassay magnetic nanoparticle probes dynamic light scattering human chorionicgonadotropin |
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