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| 傅里叶变换红外光谱法测定当归果胶多糖的酯化度 | |
| 引用本文： | 孙元琳,崔武卫,顾小红,汤坚,申瑞玲.傅里叶变换红外光谱法测定当归果胶多糖的酯化度[J].光谱学与光谱分析,2009,29(3):682-685. |
| 作者姓名： | 孙元琳 崔武卫 顾小红 汤坚 申瑞玲 |
| 作者单位： | 1. 运城学院生命科学系，山西 运城 044000 2. 加拿大农业部食品研究所，安大略 湖州 N1G 5C9 3. 江南大学食品科学与技术国家重点实验室，江苏 无锡 214122 4. 郑州轻工业学院食品与生物工程学院，河南 郑州 450002 |
| 基金项目： | 国家自然科学基金，山西省青年科研基金 |
| 摘 要： | 应用红外光谱技术建立了一种对果胶多糖酯化度(DE)进行快速定量分析的方法。以当归果胶多糖为测试样品，采用傅里叶变换红外光谱仪(FTIR)建立了DE与A1 730/(A1 730+A1 630)之间的线性回归方程，相关系数为0.985 2，表明果胶多糖的DE与其在1 730和1 630 cm-1处的吸收峰面积之间有良好的线性关系。经测定，从当归中提取的果胶多糖粗品和纯品的酯化度介于42.36%～54.06%之间。将FTIR法与传统的化学滴定法进行比较，两种方法间相对误差为7%，并具有良好的重现性，且与传统方法相比具有样品用量少、操作简单、快捷、灵敏度高等特点。FTIR测定果胶多糖酯化度具有可行性，可成为替代化学滴定法的一种的快速易行而准确的方法。 |
| 关 键 词： | FTIR 果胶多糖 酯化度 当归 |
| 收稿时间： | 2007-12-06 |
Determination of Degree of Esterification in Pectic Polysaccharides from Angelica Sinensis(Oliv.) Diels by Fourier Transform Infrared Spectroscopy |
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| SUN Yuan-lin,CUI Wu-wei,GU Xiao-hong,TANG Jian,SHEN Rui-ling.Determination of Degree of Esterification in Pectic Polysaccharides from Angelica Sinensis(Oliv.) Diels by Fourier Transform Infrared Spectroscopy[J].Spectroscopy and Spectral Analysis,2009,29(3):682-685. | |
| Authors: | SUN Yuan-lin CUI Wu-wei GU Xiao-hong TANG Jian SHEN Rui-ling |
| Institution: | 1. Department of Life Science, Yuncheng University, Yuncheng 044000, China2. Food Research Program, Agriculture and Agri-Food Canada, Ontario, Canada N1G 5C93. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China4. College of Food and Biological Engineering, Zhengzhou Institute of Light Industry, Zhengzhou 450002, China |
| Abstract: | An infrared spectroscopy method for rapid quantitative determination of the degree of esterification(DE) of pectic polysaccharides was developed.Taking pectic polysaccharides from angelica sinensis as samples,the calibration curve was established between DE and the ratio of A1 730/(A1 730+A1 630).The square of the linear correlation coefficient was 0.822,indicating a good linear relationship between the DE and the absorbent area at 1 730 and 1 630 cm-1.Using this method,the DE of pectic polysaccharides from... |
| Keywords: | FTIR |
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