Separation of chiral pharmaceuticals using ultrahigh pressure liquid chromatography |
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| Authors: | Y Xiang N Wu J A Lippert M L Lee |
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| Institution: | (1) Department of Chemistry and Biochemistry, Brigham Young University, 84602-5700 Provo, UT, USA;(2) Merck Research Laboratories, Analytical Research Department, 07065 Rahway, NJ, USA;(3) Department of Chemistry, Weber State University, 84408-2503 Ogden, UT, USA |
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| Abstract: | Summary Ultrahigh pressure liquid chromatography was demonstrated for fast and efficient chiral separations. Capillary columns approximately
13–24 cm in length packed with nonporous 1.0μm C6-modified silical particles were used. β-Cyclodextrin (β-CD) and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) were added to the
mobile phase as modifiers to produce transient diastereomeric complexes with the analytes. Pressures up to ≈42,000 psi were
applied, and efficiencies in excess of 200,000 plates m−1 were obtained for separations that were accomplished in less than 2 minutes. |
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| Keywords: | Column liquid chromatography Ultrahigh pressure chromatography Chiral drugs β -Cyclodextrin 2-Hydroxypropyl-β -cyclodextrin |
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