| 萘乙二胺法测定色氨酸及应用于DL-色氨酸拆分过程 |
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| 引用本文： | 曹飞,于文涛,武红丽,孟涛,韦萍.萘乙二胺法测定色氨酸及应用于DL-色氨酸拆分过程[J].分析试验室,2005,24(8):70-72. |
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| 作者姓名： | 曹飞 于文涛 武红丽 孟涛 韦萍 |
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| 作者单位： | 南京工业大学制药与生命科学学院,南京,210009;南京工业大学制药与生命科学学院,南京,210009;南京工业大学制药与生命科学学院,南京,210009;南京工业大学制药与生命科学学院,南京,210009;南京工业大学制药与生命科学学院,南京,210009 |
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| 摘 要： | 在酸性条件下,萘乙二胺与色氨酸形成偶氮化合物,最大吸收波长在545nm处。100倍的丝氨酸、丙氨酸、天冬氨酸、精氨酸、苯丙氨酸、酪氨酸及10倍底物N-乙酰-色氨酸均不干扰显色,甲硫氨酸对显色影响较大,相对误差达到23.9%;拆分过程所需的钴离子和10倍量的酶蛋白对显色反应也没有影响,相对误差可控制在10%以内。采用萘乙二胺法检测色氨酸更适合于色氨酸拆分过程的检测,便于控制拆分过程。
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| 关 键 词： | 萘乙二胺 色氨酸 氨基酰化酶 分光光度法 |
| 文章编号： | 1000-0720（2005）08-0070-03 |
| 收稿时间： | 2004-09-08 |
| 修稿时间： | 2004-09-082004-10-25 |
N-(1-naphthyl )-ethylenediamine spectrophotometric method for determination of tryptophan in a resolution process |
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| Cao Fei,YU Wen-tao,WU Hong-li,MENG Tao,WEI Ping.N-(1-naphthyl )-ethylenediamine spectrophotometric method for determination of tryptophan in a resolution process[J].Chinese Journal of Analysis Laboratory,2005,24(8):70-72. |
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| Authors: | Cao Fei YU Wen-tao WU Hong-li MENG Tao WEI Ping |
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| Abstract: | Under acidic condition, tryptophan is coupled with N-( 1-naphthyl )-ethylenediamine. The azo com- pound has maximum absorption at 545 nm. 100 times serine, alanine, asparatic acid, arginine, pheny- lanaline, tyrosine, and 10 times substrate, enzyme, Co2 in the resolution process did not interfered with the coloring reaction. Metionine mainly interfered with col- oring reaction. A method for the determination of trypto- phan in the resolution process by N-( 1-naphthyl )- ethylenediamine spectrophotometry was proposed. It is suitable for detecting the resolution process of trypto- phan. |
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| Keywords: | N-( 1-naphthyl )-ethylenediamine Trypto- phan Aminocaylase Spectrophotometry |
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