| 荧光光谱法研究共存物对白杨素与BSA相互作用的影响 |
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| 引用本文： | 陈秀霞,张国文.荧光光谱法研究共存物对白杨素与BSA相互作用的影响[J].分析科学学报,2009,25(6). |
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| 作者姓名： | 陈秀霞 张国文 |
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| 作者单位： | 南昌大学食品科学与技术国家重点实验室,江西南昌,330047 |
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| 基金项目： | 教育部长江学者和创新团队发展计划项目，江西省教育厅科技计划项目，江西省自然科学基金项目，南昌大学食品科学与技术国家重点实验室项目 |
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| 摘 要： | 运用荧光光谱法研究了共存物亚硝酸钠、葡萄糖或维生素C对白杨素(CHR)与牛血清白蛋白(BSA)相互作用的影响.结果表明:无共存物时,白杨素对BSA的荧光猝灭过程为静态猝灭,结合常数K值为10~3～10~4数量级,结合位点数n近似等于1,分子间相互作用力以疏水作用力为主;亚硝酸钠、葡萄糖、维生素C分别参与下,白杨素对BSA的荧光猝灭类型由静态猝灭转变为动态猝灭,葡萄糖的参与使白杨素与BSA之间作用力类型由疏水作用力转为氢键与范德华力,亚硝酸钠或维生素C的分别存在不影响白杨素与BSA的作用力类型;三种外加试剂的单独参与均使得白杨素与BSA的结合常数明显增大,结合位点数略有增加,但仍维持在1左右.初步探讨了共存物影响白杨素与BSA结合的可能方式.
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| 关 键 词： | 白杨素 牛血清白蛋白 共存物 荧光光谱 |
Effect of Coexisting Substances on the Interaction between Bovine Serum Albumin and Chrysin Investigated by Fluorescence Spectroscopy |
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| CHEN Xiu-xia,ZHANG Guo-wen.Effect of Coexisting Substances on the Interaction between Bovine Serum Albumin and Chrysin Investigated by Fluorescence Spectroscopy[J].Journal of Analytical Science,2009,25(6). |
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| Authors: | CHEN Xiu-xia ZHANG Guo-wen |
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| Institution: | CHEN Xiu-xia,ZHANG Guo-wen*(State Key Laboratory of Food Science and Technique,Nanchang University,Nanchang 330047) |
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| Abstract: | The effect of coexisting substances(NaNO2,glucose or vitamin C) on the interaction between bovine serum albumin(BSA) and chrysin(CHR) was investigated by UV-Vis and fluorescence spectroscopy.Results obtained from fluorescence spectra indicated that CHR had a strong ability to quench the intrinsic fluorescence of BSA through a static quenching procedure in the absence of coexisting substances.The magnitude of the binding constant K in CHR-BSA system was about 103~104 and the number of binding sites of chrysi... |
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| Keywords: | Chrysin Bovine serum albumin Coexisting substance Fluorescence spectroscopy |
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