Conjugation reactions in the preparations of quantum dot-based immunoluminescent probes for analysis of proteins by capillary electrophoresis |
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Authors: | M Lišková I Voráčová K Klepárník V Hezinová J Přikryl F Foret |
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Institution: | (1) Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, 602 00 Brno, Czech Republic; |
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Abstract: | A number of biologically important molecules, such as DNA, proteins, and antibodies, are routinely conjugated with fluorescent
tags for high-sensitivity analyses. Here, the application of quantum dots in the place of bright and size-tunable luminophores
is studied. Several selected bioconjugation reactions via zero-length cross-linkers, long-chain linkers, and oriented methods
for linking of quantum dots with proteins were tested. Anti-ovalbumin, anti-proliferating cell nuclear antigen, anti-hemagglutinin,
and anti-CD3 membrane protein as model antibodies and annexin V were used as high-specificity selectors. The reaction yield
and efficiency of the prepared immunoluminescent probes were tested by capillary zone electrophoresis with laser-induced fluorescence
detection. |
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