水汽界面二维银颗粒表面上的单分子拉曼光谱检测

随着各种超灵敏分析仪器的发展 ,已经可以在低温固体中、室温液体中和电介质表面检测、鉴定单分子及其动力学行为 .这种新进展为科学家在分析化学、分子生物学和纳米结构材料等各种学科的应用开辟了许多新的视窗 .单分子谱学的研究在基础科学和应用科学方面引起了人们广泛的兴趣 .人们不仅希望能够“看到”单分子 ,而且希望了解单分子的物理化学行为 .在各种超灵敏检测技术中 ,拉曼光谱成为一种重要的技术 .由于原子力显微等微区技术的发展 ,并结合高灵敏度检测技术的进步 ,拉曼光谱已经发展成为一种检测灵敏度可以达到分子级的检测技术 1,…

The Raman Detection of Single Molecule at the Aggregated Silver Particle Films on Air-water Interface

We have found that the silver colloids, which usually used as SERS active substrate, are inclined to aggregation on air water interface and forming a two dimension silver particle films. The formed silver particle films exhibit much higher surface Raman enhancement factor than silver colloids, and the power used on silver particle films is only about 1% of that on silver colloids. Combining with resonance Raman enhancement technique, we realize single molecular Raman detection under the common conditions by using this silver particle films as SERS active substrate.