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Detection of <Emphasis Type="Italic">N</Emphasis>-monomethyl-lysine generated by metabolic transmethylation |
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| Authors: | |
| Institution: | (1) Department of Pharmacology and Pharmacotherapy, Semmelweis University, 1089 Budapest, Nagyvárad tér 4, Hungary;(2) EGIS Pharmaceutical Works, Budapest, Hungary;(3) Department of Pharmaceutics, Semmelweis University, Budapest, Hungary;(4) Institute of Isotopes Co. Ltd., Budapest, Hungary;(5) Central Isotope Laboratory, Semmelweis University, Budapest, Hungary;(6) Department of Pharmacology & Therapeutics, Faculty of Medicine & Health Sciences, United Arab Emirates University, P.O.Box 17666, Al Ain, United Arab Emirates |
| Abstract: | Administration of radiolabelled deprenyl to rats resulted in the urinary elimination of a 14C-labelled N -monomethyl-lysine. An increased level of N-monomethyl-lysine was found following an oral dose of another drug, also containing an N-methyl group. The urine sample was treated with 9-fluorenylmethoxycarbonyl chloride and then subjected to high-performance liquid chromatography (HPLC); the radioactive fraction was identified as N-monomethyl-lysine by using HPLC-MS in electrospray mode. Identification of N-monomethyl-lysine in the radioactive fraction gives experimental proof of transmethylation from a well-known drug to an endogenous compound. |
| Keywords: | HPLC HPLC/MS/MS Methylation N-Monomethyl-lysine Deprenyl Urinary |
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