Analysis of Nitric Oxide from Chemical Donors Using CMOS Platinum Microelectrodes |
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| Authors: | Rachel M. Feeny John B. Wydallis Tom Chen Stuart Tobet Melissa M. Reynolds Charles S. Henry |
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| Affiliation: | 1. Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523‐1872 phone: 1‐970‐491‐2852;2. fax: 1‐970‐491‐1801;3. Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado 80523;4. Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523;5. Department of Chemical & Biological Engineering, Colorado State University, Fort Collins, Colorado 80523 |
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| Abstract: | Electrochemical detection of NO generated from chemical donors is reported. Because NO is an important biological messenger, many donor sources and detection methods have been developed. Few reports have characterized NO donors using electrochemistry despite electrochemical techniques being sensitive and selective. Here, a CMOS platinum microelectrode array is interfaced with a microfluidic device for the electrochemical analysis of NO from (Z)‐1‐[N‐(2‐aminoethyl)‐N‐(2‐ammonioethyl)amino]diazen‐1‐ium‐1,2‐diolate (DETA/NO). The donor parent amine fouls the electrode, resulting in substantial signal loss, but an electrochemical cleaning method was developed that substantially reduces fouling and allows detection of NO between 90 nM and 1 µM. |
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| Keywords: | Biosensors CMOS Electrode cleaning Neurotransmitters Nitric oxide |
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