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A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles |
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| Authors: | Ezat Hamidi‐Asl Jahan Bakhsh Raoof Mohammad Saeid Hejazi Simin Sharifi Seyed Mahdi Golabi Ilaria Palchetti Marco Mascini |
| Institution: | 1. Università degli Studi di Firenze, Dipartimento di Chimica, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy;2. Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran;3. Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran;4. Drug Applied Research Center and Pharmaceutical Nanotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;5. Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, Tabriz University, Tabriz, Iran tel: +98?11?35302392;6. fax: +98?11?35302350;7. Istituto Nazionale Biostrutture e Biosistemi, Villa delle Medaglie D'Oro, 305, 00136 Roma |
| Abstract: | The development of an electrochemical genosensor involving DNA biotinylated capture probe immobilized on streptavidin coated paramagnetic beads and microfluidic based platform for the detection of P53 gene PCR product is reported. The novelty of this work is the combination of a sensitive electrochemical platform and a proper microfluidic system with a simple and effective enzyme signal amplification technology, ELISA, for detection of target DNA sequence and single nucleotide mutation in p53 tumor suppressor gene sequence. The biosensor has been applied to detect the PCR amplified samples and the results shows that it can discriminate successfully perfect matched DNA from mutant form. |
| Keywords: | Magnetic beads ELISA Sandwich hybridization Microfluidic platform PCR products |