Impact of Electrochemical Impedance Spectroscopy Experimental Variables on Adsorbed Protein Films,as Illustrated by Bovine Serum Albumin |
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Authors: | Michelle A MacDonald Heather A Andreas |
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Institution: | Department of Chemistry, Dalhousie University, Halifax, NS, Canada B3H 4R2 |
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Abstract: | Electrochemical Impedance Spectroscopy (EIS) is often used to examine protein films, such as Bovine Serum Albumin (BSA) films formed by incubation on platinum electrodes. Literature EIS experiments often vary in terms of whether blank EIS spectra are collected prior to film formation, whether a redox probe like ferricyanide is used, and the potential at which the EIS spectrum is collected. These experimental differences may lead to significant differences in the protein film and the results measured. This paper examines the impact of these factors on the Nyquist plots recorded for BSA films formed by adsorption on platinum electrodes. |
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Keywords: | EIS variables Applied potential Ferricyanide Competitive adsorption Platinum electrode |
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