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Assessment of the Antioxidant Capacity of Standard Compounds and Fruit Juices by a Newly Developed Electrochemical Method: Comparative Study with Results from Other Analytical Methods
Authors:Isacco Gualandi  Luca Ferraro  Pietro Matteucci  Domenica Tonelli
Institution:Department of Industrial Chemistry ”Toso Montanari”, University of Bologna, Viale del Risorgimento 4, 40136 Bologna, Italy
Abstract:The antioxidant capacity (AOC) of some standard compounds and commercial beverages was determined using an electroanalytical method which measures the OH radical scavenging ability (OH‐RSC). It is based on the degradation of a thin polyphenol film electrodeposited on glassy carbon following the attack by OH radicals produced by hydrogen peroxide photolysis. The degradation is prevented in presence of increasing concentrations of antioxidants. The same samples were also analyzed by DPPH, ABTS, and ORAC assays. The OH‐RSC method provided AOC values highly correlated with those resulting from the application of ORAC assay for both juices (R=0.90) and standard antioxidants (R=0.96).
Keywords:Antioxidant capacity  Electrochemical assay  OH radical scavenging ability  Pure antioxidants  Fruit juices
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