Detection of p53 Gene by Using Genomagnetic Assay Combined with Carbon Nanotube Modified Disposable Sensor Technology |
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| Authors: | Gulsah Congur Medard Plucnara Arzum Erdem Miroslav Fojta |
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| Institution: | 1. Ege University, Faculty of Pharmacy, Analytical Chemistry Department, 35100 Bornova, Izmir, Turkey phone: +90‐232‐311?5131;2. fax: +90‐232‐388?5258;3. Institute of Biophysics, Academy of Sciences of the Czech Republic v.v.i., Kralovopolska 135, CZ‐61265 Brno, Czech Republic phone: +420?5?41517197;4. fax: +420?5?41211293;5. Central European Institute of Technology, Masaryk University Kamenice 753/5, CZ‐625 00 Brno, Czech Republic |
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| Abstract: | Electrochemical monitoring of DNA hybridization related to p53 gene sequence was investigated using genomagnetic assay combined with single walled carbon nanotube (SWCNT) modified pencil graphite electrodes (PGEs). The hybridization was performed either at magnetic beads (MB) surface or in solution. The enhanced guanine signal was obtained using SWCNT‐PGEs compared to one obtained by unmodified PGEs. The selectivity of genomagnetic assay was tested under optimum conditions. The DLs were calculated as 0.88 µM and 0.11 µM for hybridization performed at MB surface and solution, respectively. This selective, practical and cost effective genomagnetic assay combined with SWCNT‐PGEs is reported herein for the first time. |
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| Keywords: | p53 Gene Carbon nanotubes Magnetic particles DNA hybridization Differential pulse voltammetry |
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