A Novel DNA Probe Based on Molecularly Imprinted Polymer Modified Electrode for the Electrochemical Monitoring of DNA |
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| Authors: | Mihrican Muti Mert Soysal Fatma Merve Nacak Kübra Gençdağ A. Ersin Karagözler |
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| Affiliation: | 1. Adnan Menderes University, Faculty of Arts and Sciences, Chemistry Department, 09010 Ayd?n, Turkey tel.: +90‐256‐218 2000, ext. 2209;2. fax:+ 90‐256‐213 5379;3. tel.: +90‐256‐218 2000, ext. 1898;4. fax: +90‐256‐213 5379;5. Adnan Menderes University, Ayd?n Health Services Voc. School, Med. Lab. Prog, 09100 Ayd?n, Turkey |
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| Abstract: | A DNA probe that was based on methylene blue (MB) imprinted polyvinyl pyridine polymer (MIP) modified carbon paste electrodes were developed for the first time for electrochemical monitoring of DNA. Probes were built up by adsorbing MB onto modified electrodes prior to DNA immobilization. It was shown that DNA strongly immobilizes on MIP modified electrodes when MB was adsorbed in advance of DNA immobilization. The performance of the MB imprinted polymer modified carbon paste electrodes (MIP‐CPE) to rebind the template molecule (MB) were compared to those of control polymer modified (non‐imprinted polymer NIP‐CPE) and bare (CPE) electrodes. Electrochemical signal resulting from the oxidation of guanine moiety of the immobilized probe DNA was high enough on the constructed platform, implicating that probes of this kind could be favorably used for DNA analysis. These probes exhibited high selectivity for its complementary DNA sequences (target). HBV‐DNA hybridization was studied to evaluate the selectivity of the probes for complementary, non‐complementary and mismatch sequences. The detection limit of the probe for the target DNA was 8.72 µg/mL (1.38 µM), which was better than those attained by some earlier DNA sensor studies. |
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| Keywords: | Molecularly imprinting polymer Methylene blue Modified electrode Electrochemistry DNA sensor |
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