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Galactose Oxidase/Prussian Blue Based Biosensors |
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| Authors: | Franck Charmantray Nadia Touisni Laurence Hecquet Thierry Noguer Christine Mousty |
| Institution: | 1. Clermont Université, Université Blaise Pascal, Institut de Chimie de Clermont‐Ferrand, BP 10448, F‐63000 Clermont‐Ferrand, France;2. CNRS, UMR 6296, ICCF, F‐63171 Aubière, France;3. Université de Perpignan Via Domitia, IMAGES EA4218, Batiment S, 52 Avenue Paul Alduy, F‐66860 Perpignan cedex, France |
| Abstract: | This paper describes the development of an amperometric biosensor based on galactose oxidase (GAOx) immobilization within a laponite clay film deposited on Carbon Screen‐Printed Electrodes modified by electrodeposited Prussian Blue and coated with poly‐(O‐phenylenediamine) (PPD/PB/CSPEs). Amperometric performances of GAOx@laponite/PPD/PB/CSPEs bioelectrodes were determined using several GAOx substrates. Using these modified electrodes the reduction of enzymatically generated hydrogen peroxide was performed at ?0.2 V vs. Ag‐AgCl. In an initial attempt, E.Coli transketolase activity on its immobilized form was followed using a bienzymatic GAOx‐TK biosensor. |
| Keywords: | Galactose oxidase Transketolase Prussian Blue Screen‐printed electrode Clays LDH |