Fluorescent 1,10-phenanthroline-containing oligonucleotides distinguish between perfect and mismatched base pairing |
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| Authors: | Hurley Dennis J Seaman Susan E Mazura Jan C Tor Yitzhak |
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| Institution: | Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0358, USA. |
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| Abstract: | structure: see text] A fluorescent deoxyuridine analogue is sensitive to the polarity of its environment and exhibits a distinct emission profile in single- vs double-stranded oligonucleotides. Emission-monitored denaturation curves of internally modified dU(phen) duplexes are characteristic of the base opposite dU(phen) and distinguish between perfect and mismatched complementary oligonucleotides. |
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