格拉司琼与磷钨杂多酸相互作用的共振光散射及分析应用

在pH 2.2 BR缓冲介质中, 磷钨杂多酸(PTA)与格拉司琼(GSN)相互作用形成离子缔合物, 不仅引起吸收光谱的变化, 还导致共振散射光谱(RLS)的显著增强并产生新的RLS光谱, 最大RLS峰位于333 nm附近, 其RLS增强程度与格拉司琼浓度成线性关系, 检出限和线性范围分别为12 ng/mL和0.04～3.0 μg/mL。文中研究了反应产物的吸收和RLS光谱特征, 优化反应条件的影响, 据此发展了以磷钨杂多酸为光谱探针的灵敏、简便、快速测定格拉司琼的新方法。将方法用于血清中格拉司琼含量的快速定, 结果满意。讨论了离子缔合反应和RLS增强机理。

Resonance Light Scattering of the Interaction of Granisetron with Phosphotungstic Acid and its Analytical Application

In pH 2.2 BR buffer solution,Phosphotungstic acid(PTA) and granisetron(GSN) interacted to form ion-association complex,which not only caused the change of absorption spectra,but also led to the resonance light scattering(RLS) markedly increased and the new RLS spectrum appeared,and the maximum RLS peak was located in the near of 333 nm.There was a linear relation between the enhancement degree of the scattering intensity and the concentration of granisetron,and the detection limit and the linear range were 12 ng/mL and 0.04~3.0 μg/mL,respectively.The absorption and RLS spectral characteristics of the system were studied;the optimum reaction conditions were discussed.Phosphotungstic acid was chosed as spectral probe,and a new,sensitive,simple and rapid method for determining granisetron was developed.The method had been applied to determination granisetron in human serum samples with satisfactory results.The reaction mechanism and reasons for RLS enhancement were discussed.