Use of design of experiments to optimize high-throughput semipreparative LC and LC/MS methods |
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Authors: | Cole Derek C Pagano Nicholas Kelly Michael F Ellingboe John |
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Affiliation: | Chemical & Screening Sciences, Wyeth Research, 401 N. Middletown Road, Pearl River, New York 10965, USA. coledc@wyeth.com |
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Abstract: | This paper describes an investigation into decreasing the run time for high-throughput semipreparative RP-HPLC methods without compromising the resolution. Experimental design was used to devise a small set of experiments in which factors, including solvent flow rate, solvent/column temperature, at-column dilution, and run time were varied systematically. The results were analyzed by means of multiple regression and partial least squares to generate a model relating the factors to the results, showing which factors are important. The model was then used to determine the optimal conditions. |
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