| 固定化金属螯合亲和膜制备及其性能研究 |
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| 引用本文： | 魏琪,姚汝华,鲍时翔.固定化金属螯合亲和膜制备及其性能研究[J].华南理工大学学报(自然科学版),2000,28(12):82-87. |
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| 作者姓名： | 魏琪 姚汝华 鲍时翔 |
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| 作者单位： | 1. 华南理工大学,食品与生物工程学院,广东,广州,510640
2. 中国热带农科院,生物技术国家重点实验室,海南,海口,571101 |
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| 基金项目： | 中国科学院资助项目,29606004, |
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| 摘 要： | 将纤维素滤纸经碱处理,环氧活化,配基偶联和固定化Cu2 后制得了大 孔纤维素亲和膜,检验了亲和膜的流速与负压,等温吸附,保存方法及使用次数等亲和膜性能指标,设计了一种解决金属离子泄漏问题的新方法,得到的结果明显优于传统的凝胶柱,以上结果表明,普通纤维素滤纸可以制成性能优良的亲和膜色谱介质。
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| 关 键 词： | 亲和膜 亲和色谱 蛋白质纯化 固定化金属螯合和膜 |
| 修稿时间： | 2000-03-28 |
Preparation of Immobilized Metal-chelated Affinity Chromatographic Membranes and Their Properties Study |
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| WEI Qi,YAO Ru-hua,BAO Shi-xiang.Preparation of Immobilized Metal-chelated Affinity Chromatographic Membranes and Their Properties Study[J].Journal of South China University of Technology(Natural Science Edition),2000,28(12):82-87. |
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| Authors: | WEI Qi YAO Ru-hua BAO Shi-xiang |
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| Abstract: | High quality affinity membranes were prepared by reaction of cellulose analysis filter papers with epichorohydrin. After coupling iminodiacetic acid and immobilizing Cu2+ onto these membranes, the Immobilized Metal-Chelated Affinity Chromatographic (IMAC) membranes were achieved. The properties of IMAC membranes such as flow-rate and backpressure, isotherm equilibrium adsorption, conservation methods and stability were evaluated. A new method was designed to successfully resolve metal ion leaking and obtained much better result than conventional chelating sepharose gel column. Their promising properties proved that they could be used to separate and purify biomacromolecules. |
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| Keywords: | affinity membrane affinity chromatography protein purification |
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