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Theory of reflectance measurements in quantitative evaluation in TLC and HPTLC. 1. Multilayer model of reflectance and transmittance
Authors:S. Ebel  P. Post
Abstract:In the approach described in this paper, the layer of adsorbent is divided into n sublayers. The radiation density in each sublayer is given by the sum of the transmittance and the reflectance of the adjacent sublayers. Absorption of light by the layer or the substance will diminish this radiation density. Depending upon the factors of reflectance and transmittance some light will pass to the adjacent sublayers. Two polynomials are derived from this readily understood formulation to calculate the reflectance or the transmittance of a layer of finite thickness. The algorithms given describe the reflectance and transmittance encountered in practical measurements with large amounts of substance per spot, as well as with small amounts. There are no restrictions with respect to nonideal scattering or reflection by the adsorbent layer support.
Keywords:Thin-layer chromatography  Quantitation model  Short computer program in BASIC given
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