FACTORS INFLUENCING THE NEAR-UV EFFECT ON DNA IN THE PRESENCE OF 8-METHOXYPSORALEN: RENATURABILITY OF DNA. |
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| Authors: | Hitoshi Fujita Miyaharu Sano Kenshi Suzuki |
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| Institution: | Department of Molecular Biology, Tokai University School of Medicine Bohseidai, Isehara-shi, Kanagawa-ken, 259–11, Japan. |
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| Abstract: | Factors affecting the near-UV induced reaction of calf thymus DNA in the presence of 8-methoxypsoralen were examined. DNA became more renaturable with increasing concentration of 8-methoxypsoralen and fluence. Existence of paramagnetic ions, Mn2+, Co2+, or Ni2+, largely repressed the photoreaction. It was ascertained that the highest renaturability was attained by the illumination with light between 310 and 345 nm. |
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