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| 维生素B_6与牛血清白蛋白相互作用的液滴荧光法研究 | |
| 引用本文： | 柴化鹏,冯锋,陈泽忠,梁文娟,白云峰,董川,双少敏.维生素B_6与牛血清白蛋白相互作用的液滴荧光法研究[J].分析试验室,2009,28(10). |
| 作者姓名： | 柴化鹏 冯锋 陈泽忠 梁文娟 白云峰 董川 双少敏 |
| 作者单位： | 1. 山西大学化学化工学院,太原,030006 2. 山西大学化学化工学院,太原,030006;山西大同大学化学化工学院,大同,037009 3. 山西大同大学化学化工学院,大同,037009 |
| 基金项目： | 国家自然科学基金，山西省自然科学基金 |
| 摘 要： | 利用荧光及紫外光谱法研究了生理pH条件下水溶液体系中维生素B6与牛血清白蛋白(BSA)的相互作用机制.维生素B6对牛血清白蛋白的荧光有较强的猝灭作用,其猝灭类型为静态猝灭.根据F(o)rster的偶极-偶极非辐射能量转移理论算出供体-受体的结合距离为2.72 nm.由Linewear-Burk方程求出不同温度下反应时复合物的形成常数KLB和结合位点数n及对应温度下结合反应的热力学参数,证明二者主要靠氢键和范德华力结合.同时采用同步荧光分析技术,对蛋白质与药物结合时构象的变化进行了探讨. |
| 关 键 词： | 液滴荧光 维生素B6 牛血清白蛋白 结合反应 |
Investigation on the interaction between VB_6 and bovine serum albumin by fluorescence analysis based on liquid drop |
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| CHAI Hua-peng,FENG Feng,CHEN Ze-zhong,LIANG Wen-juan,BAI Yun-feng,DONG Chuan,SHUANG Shao-min.Investigation on the interaction between VB_6 and bovine serum albumin by fluorescence analysis based on liquid drop[J].Chinese Journal of Analysis Laboratory,2009,28(10). | |
| Authors: | CHAI Hua-peng FENG Feng CHEN Ze-zhong LIANG Wen-juan BAI Yun-feng DONG Chuan SHUANG Shao-min |
| Institution: | 1.College of Chemistry and Chemical Engineering;Shanxi University;Taiyuan 030006;2.Institute of Organic Chemistry;Shanxi Datong University;Datong 037009 |
| Abstract: | Under the physiological conditions of the body,the interaction between VB6 and bovine serum albumin(BSA) was investigated by fluorescence spectrometry and ultraviolet absorption(UV) spectrometry based on liquid drop.The experimental results showed that VB6 quenched the fluorescence of BSA by forming a VB6-BSA complex.According to the relation between thermodynamic parameters and the interaction force,VB6 depended principally on the dispersive interaction to bind with BSA.The results showed that the quenchin... |
| Keywords: | Fluorescence of liquid drop VB6 BSA Binding reaction |
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