Photocontrol of lactate dehydrogenase-spiropyran collagen membrane |
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| Authors: | Yoshiaki Nakamoto Isao Karube Seikichi Terawaki Shuichi Suzuki |
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| Institution: | 1. Department of Industrial Chemistry, University of Kanazawa, Kodatsuno, Kanazawa-city, Ishikawa-Ken, Japan
2. Research Laboratory of Resources Utilization Tokyo Institute of Technology Ookayama Meguro-ku, Tokyo, Japan
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| Abstract: | Collagen fibrils were modified with β-l-3,3-dimethyl-6′-nitrospiro-(indoline-2,2′-2H-benzopyran)] propionic anhydride. The spiropyran collagen membrane showed reverse photochromism. Lactate dehydrogenase (LDH, E.C. 1.1.1.27) was entrapped in the spiropyran collagen membrane. The activity of the LDH-spiropyran collagen membrane decreased under visible-light irradiation, and then increased again after incubation in the dark. The optimum pH of the LDH-spiropyran collagen membrane was displaced toward lower pH values under visible-light irradiation. The activity change of the LDH-spiropyran collagen membrane under visible-light irradiation depended on the LDH content. |
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