Reduction in the antigen-binding in polyacrylamide gel |
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Authors: | T B Greenland Y Manuel |
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Institution: | 1. Institut Pasteur de Lyon, 77 rue Pasteur, 69007, Lyons, France
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Abstract: | Under conditions of antibody excess, antibodies that had been immobilized by physical entrapment in a polyacrylamide gel matrix consistently removed less radiolabeled microprotein antigen from solution than did the same antibodies chemically conjugated to Sepharose beads. This reaction is believed to relate to the difficulty of formation of secondary antibody-antigen lattices when the molecules are held in “cells” in the polymer matrix. |
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