FTIR用于变性蛋白质二级结构的研究进展

蛋白质是最重要的生命物质之一，有关蛋白质的各类研究也是人们比较感兴趣的课题。蛋白质的二级结构在维持蛋白质生理活性过程中发挥着重要的作用。随着科技的发展和研究方法的改进，对变性蛋白质微观结构的研究也越来越深入。近年来，国内外学者利用各种物理和化学方法对其作了大量的工作，而傅里叶红外光谱法(FTIR)以其独特的优越性，在研究蛋白质二级结构中发挥着不可替代的作用。文章就这方面的研究进行了初步概述，重点介绍了变性蛋白质二级结构的红外光谱学研究内容、谱学特点及其分析方法的研究进展。

Progress in Study of Secondary Structure of Denaturized Protein by FTIR

Protein is one of the most important living matters, and researches relatedto it have interested so many scientific workers. The secondary structure of protein plays a crucial role in maintaining protein's physiological activity. With the development of technology and the improvement of research technique, there are more and more in-depth studies on protein's microstructure. In recent years, many research works in this field have been done with different kinds of physical and chemical methods and Fourier transform infrared spectroscopy(FTIR)holds an unshaken position due to its unique superiority. The present paper is a general review of the related studies all over the world, and the assignments of the major infrared absorption bands of denaturized protein are introduced, and the spectroscopic features and the analytical methods are mainly reviewed.