Sensitive Determination of Adrenaline by Means of a Flow-Through Solid Phase UV Spectrophotometric Sensing Device |
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| Authors: | A Ruiz Medina M L Fernández de Córdova A Molina Díaz |
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| Institution: | (1) Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Paraje Las Lagunillas, s/n, E-23071 Jaén, Spain, ES |
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| Abstract: | A very sensitive flow-through optosensor with solid phase UV spectroscopic detection is proposed for the direct determination
of adrenaline without prior derivatization. Sample is transported by the carrier stream (0.05 M NaCl/0.01 M NaOH) to the sensing
microzone composed of Sephadex QAE A-25 resin placed in a flow-through cell, and its intrinsic absorbance is monitored at
287 nm. After development of the analytical signal adrenaline is easily and quickly desorbed from the solid support by a 0.7 M
NaCl/0.01 M NaOH eluting solution stream. Adrenaline can be determined in the range 1–12 μg/ml, the detection limit being
0.17 μg/ml. The RSD (10 replicates) and sample throughput are 1.4% and 12 per hour, respectively. The procedure has been successfully
applied to the determination of adrenaline in different medical formulations.
Received May 26, 1999. Revision February 3, 2000 |
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| Keywords: | : Adrenaline flow-through optosensor pharmaceuticals |
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