吖啶酯标记的化学发光免疫分析优化参数评价

以睾酮-3-羧甲氧基肟-牛血清白蛋白为免疫原,免疫家兔产生抗血清,10-甲基吖啶-9-羧酸4-(N-睾酮-3-羧甲氧基肟)-氨乙基]苯酯为示踪化合物,采用平衡法建立睾酮化学发光免疫分析,数据用四参数Logistic函数处理。方法学考核标准曲线稳定,R为0.9967;ED50为82.15ng/L;批内和批间CV分别为5.78%和3.3%;平均回收率为107.28%;可测范围为19.3～264.6ng/L,与雌酮、雌二醇、雌三醇和孕酮的交叉反应率均小于1.35%;非特异结合率为9.64%.“桥效应”分析表明同位点、“桥”结构相同亲和力最大.

EVALUATION OF OPTIMAL PARAMETERS FOR ACRIDINIUM ESTER LABELED CHEMILUMINESCENE IMMUNOASSAY

Immunogen was testosterone-3-carboxymethyl oxime-bovine serum albumin(To-3-CMO-BSA), and tracer ligand was testosterone-3-carboxymethyl oxime-4-(10-methy)-acridinium-9-carboxylate(To-3-CMO-AEPMAC). A chemiluminescene immunoassay of testosterone(To-CLIA) was established, on the equilibrium method, by raising the antiserum in rabbits against To-3-CMO-BSA. The data was processed by the four-parameters logistic function program. The intra- and inter-assay CV of To-CLIA were 5.78% and 3.30%, respectively, the recovery rate, 107.28%. The antiserum provided a linear response from 19.3 to 264.6ng/L with ED 50 of 82.15ng/L and correlation coefficient of curve fitting, 0.9967. The cross reactivity with estrone, estradiol, estriol and progesterone were less 1.35%. Non-specific bound rate was 9.64%. The highest affinity constant could be obtained by "bridge effect" analysis with same structure of the "bridge" in the same position of testosterone.