Anti-inflammatory bibenzyls from the stems of Dendrobium huoshanense via bioassay guided isolation |
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| Authors: | Qiang-Ming Li Hui Jiang Xue-Qiang Zha Li-Hua Pan Jun Duan |
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| Affiliation: | 1. School of Food Science and Engineering, Hefei University of Technology, Hefei, People’s Republic of China;2. School of Biological and Medical Engineering, Hefei University of Technology, Hefei, People’s Republic of China;3. Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, People’s Republic of China |
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| Abstract: | AbstractThe stems of Dendrobium huoshanense have long been used to prevent various diseases, including inflammatory diseases. This study was aimed to explain the anti-inflammatory effect of D. huoshanense stems in LPS-induced RAW 264.7 macrophages and to discover potential anti-inflammatory compounds. Results exhibited that D. huoshanense stems ethanol extract could significantly inhibit LPS-induced production of NO, TNF-α and IL-1β. Based on bioassay guided strategy, four bibenzyls (1–4) were isolated from D. huoshanense stems for the first time. Anti-inflammatory assay showed 1–4 could remarkably inhibit the production of NO in LPS-induced macrophages. Moreover, quantitative RT-PCR analysis displayed that the mRNA levels of iNOs, TNF-α and IL-1β could also be significantly reduced by 1–4. These results suggested that D. huoshanense stems ethanol extract and bibenzyls 1–4 might be well developed as therapeutic agent to prevent inflammatory diseases. |
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| Keywords: | Dendrobium huoshanense anti-inflammation bioassay guided bibenzyls macrophages |
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