In vitro solar protection factor,antioxidant activity,and stability of a topical formulation containing Benitaka grape (Vitis vinifera L.) peel extract |
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Authors: | Letícia Caramori Cefali Ilza Maria de Oliveria Sousa Mariana Cecchetto Figueiredo Ana Lucia Tasca Gois Ruiz Mary Ann Foglio |
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Institution: | 1. Graduate Program in Biosciences and Technology of Bioactive Products, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil;2. Graduate Program in Pharmaceutical Science, Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil;3. Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil AND Chemical, Biological and Agricultural Pluridisciplinary Research Center (CPQBA), University of Campinas (UNICAMP), Paulínia, SP, Brazil |
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Abstract: | AbstractA flavonoid enriched extract (FE) was obtained from grape peels, and in vitro SPF, antioxidant activity, and effects on cell viability of this extract were tested with the intent to develop a cosmetic product. A formulation was developed with the FE, and the stability of this mixture was evaluated in terms of pH, density, viscosity, and SPF (90-days). FE showed no cytotoxicity to human keratinocytes and an in vitro SPF of 18.56 (UV-spectrophotometry). Further, FE showed a UVA protection factor of 3.17?±?0.2, a critical wavelength of 318.0?±?0.1 and a UVA/UVB of 0.9. Antioxidant activity assays resulted in 92.08% and 86.85% of activity against DPPH and ABTS (IC50 = 296.90?±?1.2?µg/mL and 643.13?±?0.9?µg/mL), respectively. Finally, SPF of formulation with FE was 12.45. Results from the in vitro SPF and product stability tests (especially storage under refrigeration), indicate that FE is a promising compound for use as an innovative sunscreen formulation. |
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Keywords: | Benitaka grape sunscreen development flavonoids rutin |
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