Enhancement of phenolic and flavonoids compounds,antioxidant and cytotoxic effects in regenerated red cabbage by application of Zeatin |
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| Authors: | Seyed Ali Ravanfar Pooyan Mehrabanjoubani Mahdi Ebrahimi |
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| Institution: | 1. Department of Horticulture, Citrus Research and Education Center-University of Florida/IFAS, Lake Alfred, FL, USA;2. Department of Basic Science, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran;3. Department of Plant Science and Biotechnology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran |
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| Abstract: | AbstractThis study evaluated the roles of zeatin (2?mg/L) on direct organogenesis, phytochemical compounds, antioxidant activity, and cytotoxic activity in regenerated shoots of red cabbage. The results revealed that the extract of explant treated by 2?mg/L zeatin gives the highest content of total phenolics (5.18?mg gallic acid equivalent/g dry weight) and flavonoids (1.52?mg rutin equivalent/g dry weight). Moreover, HPLC and GC-MS analyses indicated that various bioactive compounds in red cabbage are significantly enhanced with increasing zeatin concentration. Besides that, antioxidant activity test showed that in vitro shooting culture using 2?mg/L zeatin displayed higher antioxidant activity in all assays (DPPH, FRAP and ABTS) compared to control with respective values of 68.12%, 73.28%, and 54.1%, respectively. Finally, the cytotoxic properties illustrated that the extracts of red cabbage explant treated by 2?mg/L zeatin exhibited the strongest cytotoxic effect towards cancer cells compared to control. |
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| Keywords: | Red cabbage Efficient plant regeneration Cytokinins Antioxidant activity |
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