Phytochemical profiling and evaluation of modified resazurin microtiter plate assay of the roots of Trillium govanianum |
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| Authors: | Kashif Maqbool Khan Satyajit D Sarker Ghazanfar Ali Khan Hammad Saleem Shujaat Ali Khan |
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| Institution: | 1. Faculty of Science, School of Pharmacy and Biomolecular Sciences, Medicinal Chemistry and Natural Products Research Group, Liverpool John Moores University
, England , UK;2. Department of Pharmacy, COMSATS University Islamabad
, Abbottabad Campus , Pakistan;3. Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences
, Lahore , Pakistan;4. amannan@cuiatd.edu.pk kashif.maqbool@uvas.edu.pk;6. Division of Medical Devices and Medicated Cosmetics, Drug Regulatory Authority
, Islamabad , Pakistan;7. Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences
, Lahore , Pakistan;8. Department of Pharmacy, COMSATS University Islamabad
, Abbottabad Campus , Pakistan |
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| Abstract: | Abstract Trillium govanianum Wall. ex D. Don (Melanthiaceae alt. Trilliaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of Trillium govanianum and its solid phase extraction (SPE) fractions by using resazurin microtiter assay (REMA) against Gram positive and Gram negative bacterial registered strains and to carry out phytochemical analysis. The remarkable amount of gallic acid equivalent phenolic and quercetin equivalent flavonoid content was manifested by MeOH extract (20.27?±?3.03?mg GAE/g DW and 9.25?±?0.50?mg QE/g DW respectively). The GC/MS analysis revealed the presence saturated and unsaturated components. Considerable level of antibacterial potential against Gram-positive bacteria (MIC: 2.5-0.009?mg/mL) than against Gram-negative bacteria (MIC: 2.5-0.165?mg/mL) were observed. The use of microtiter plates has the advantage of lower cost, fast and quantitative results. |
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| Keywords: | Trillium govanianum Melanthiaceae GC/MS SPE REMA MIC |
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