Isolation,characterization, and xanthine oxidase inhibitory activities of flavonoids from the leaves of Perilla frutescens |
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| Authors: | Yang Liu Yuxue Hou Yueyue Si Wei Wang Shuang Zhang Shiwei Sun |
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| Affiliation: | Department of Natural Medicine and Pharmacognosy, School of Pharmacy, Qingdao University, Qingdao, China |
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| Abstract: | AbstractPhytochemical investigation of the water extract from the leaves of Perilla frutescens (Lamiaceae) led to the isolation of a new flavanone, a new chalcone, and a new aurone, namely, (2S)-5,7-dimethoxy-8,4'-dihydroxyflavanone (1), 2',4'-dimethoxy-4,5',6'-trihydroxychalcone (2), and (Z)-4,6-dimethoxy-7,4'-dihydroxyaurone (3), respectively. The structures were unambiguously elucidated on the basis of spectroscopic data. And the absolute configuration of 1 was determined by analysis of electronic circular dichroism spectrum. The isolated compounds were evaluated for their inhibitory effects on xanthine oxidase in vitro. Among them, 2 showed more potent activity than the positive control allopurinol, a well-known XO inhibitor clinically used for treatment of gout. Lineweaver-Burk transformation of the inhibition kinetics data demonstrated that it was a mixed-type inhibitor. | |
| Keywords: | Perilla frutescens (L.) Britt. hyperuricemia xanthine oxidase flavanone chalcone aurone |
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