Polyphenols contents,heavy metals analysis and in vitro antibacterial activity of extracts from Cladanthus arabicus and Bubonium imbricatum of Moroccan Origin |
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Authors: | Abdellah Aghraz Ambrogina Albergamo Qada Benameur Andrea Salvo Mustapha Larhsini Mohamed Markouk |
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Affiliation: | 1. Laboratory of Biotechnology, Protection and Valorisation of Plant Ressources (URAC35 Association Unit), Cadi Ayyad University, Marrakesh, Morocco;2. Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, Messina, Italy;3. Nursing Department Faculty of Natural Sciences and Life, Abdelhamid Ibn Badis University, Mostaganem, Algeria;4. Ecole Nationale Supérieure Vétérinaire d’El-Harrach, Laboratoire de Recherche ?Santé et Production Animale?, Algiers, Algeria |
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Abstract: | AbstractAim of this study was to evaluate polyphenols and major and trace elements of Cladanthus arabicus and Bubonium imbricatum, along with their in vitro antibacterial activity against six multidrug resistant Enterobacteriaceae (Escherichia coli S33/16, E. coli S34/16, Proteus mirabilis S32/16, Klebsiella pneumoniae S12/16, Enterobacter cloacae S5/16, and Salmonella sp S12/14). UV spectrophotometry, ultra-high-performance liquid chromatography coupled to mass spectrometry and inductively coupled plasma mass spectrometry were used to evaluate total polyphenol content, quali-quantitative profile of single polyphenols and inorganic elements of the extract. The antibacterial activity was investigated by standard methods. Twelve polyphenols were identified in both plants and these were more concentrated in B. imbricatum than C. arabicus extracts. High levels of minerals, essential trace elements and tolerable levels of heavy metals (Cd, As and Pb) were found. Furthermore, the extracts showed also a strong in vitro antibacterial activity, particularly versus E. coli S33/16 (MIC, 0.125?mg ml?1). |
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Keywords: | Bubonium imbricatum Cladanthus arabicus antimicrobials enterobacteria polyphenols major and trace elements heavy metals |
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