Antifungal activity study of the monoterpene thymol against Cryptococcus neoformans

Abstract

Cryptococcus neoformans is a yeast fungus, which causes cryptococcosis, triggered by basidiospore inhalation and consequent dissemination to the central nervous system. In this study, we analyzed the antifungal action of thymol against 10 clinical strains of C. neoformans and analyzed the interaction of this monoterpene with sterols. The MICs of thymol ranged from 20 to 51?μg/ml, while the MFC values varied between 40 and 101?μg/ml. For the strains ICB-2601 and LM-39, in the presence of ergosterol, the MIC of thymol was 64?μg/ml, and in the presence of cholesterol, its MIC was 32?μg/ml. Based on the results, thymol presents antifungal action and seems to interact with ergosterol, but not with cholesterol. Complementary studies are needed to analyze its full effects.