New cytotoxic bufadienolides from the roots and rhizomes of Helleborus thibetanus Franch |
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Authors: | Yuze Li Huawei Zhang Xiaofei Liang Bei Song Xudong Zheng Rui Wang |
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Affiliation: | 1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, China;2. College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, China |
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Abstract: | AbstractThree new bufadienolides 14β, 16β-dihydroxy-3β-[β-D-glucopyranosyl-(1→6)-(β-D-glucopyranosyl)oxy]-5α-bufa-20, 22-dienolide (1), 14β-hydroxy-3β-[β-D-glucopyranosyl-(1→4)-(β-D-glucopyranosyl)oxy]-5α-bufa-20, 22-dienolide (2) and hellebrigenin-3-O-β-D-glucosyl-(1→4)-β-D-glucoside (3), together with eight known bufadienolides (4–11) were isolated from the roots and rhizomes of Helleborus thibetanus. Their structures were elucidated by extensive spectroscopic methods and acid hydrolysis. Compounds 1–7 were evaluated for their cytotoxic activity against HCT116, A549 and HepG2 tumor cell lines. Compound 1 exhibited moderate cytotoxicity against HepG2 cells with IC50 value of 15.1?±?1.72?μM. Compounds 5 and 6 exhibited moderate cytotoxicity against HCT116 cells with IC50 values of 15.12?±?0.58?μM and 13.17?±?2.34?μM, respectively. |
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Keywords: | bufadienolide glycosides cytotoxic activity Helleborus thibetanus Franch structure identification |
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