Anti-hypertensive effects of Radix Rehmanniae and its active ingredients |
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| Authors: | Chiu-Haw Chao Jue-Liang Hsu Mu-Fen Chen Ya-Hu Shih Chia-Hui Lee |
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| Institution: | 1. Department of Food Science, National Pingtung University of Science and Technology, Pingtung, Taiwan;2. Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, Taiwan;3. Research Center for Active Natural Products Development, National Pingtung University of Science and Technology, Pingtung, Taiwan;4. Research Center for Austronesian Medicine and Agriculture, National Pingtung University of Science and Technology, Pingtung, Taiwan;5. Research Center for Tropic Agriculture, National Pingtung University of Science and Technology, Pingtung, Taiwan;6. Research Center for Animal Biologics, National Pingtung University of Science and Technology, Pingtung, Taiwan |
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| Abstract: | AbstractRadix Rehmanniae (RR) is the root tuber of Rehmannia glutinosa Libosch. Herein, the methanol extracts of dried RR (DRR) and processed RR (PRR) were partitioned to obtain ethyl acetate, aqueous, and n-butanol layers. The angiotensin-I converting enzyme (ACE) inhibition test indicated that the ethyl acetate extracts of DRR (DRRE) and PRR (PRRE) show better inhibitory activity. Therefore, changes in blood pressure were tested over 24?h in spontaneously hypertensive rats, with DRR showing good anti-hypertensive activity. DRRE was further subjected to column chromatography; 28 fractions were separated and tested for ACE inhibition. Ultimately, six compounds were identified by spectral analysis, and literature comparison. Specifically, ursolic acid and oleanolic acid showed better ACE inhibition than the other compounds. This study confirmed that DRR has anti-hypertensive activity. In future, DRR’s potential as a health food should be further assessed. |
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| Keywords: | Angiotensin-I converting enzyme Radix Rehmanniae Antihypertension Spontaneously hypertensive rats Triterpene |
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